Tat transduction peptide displayed on the nucleocapsid improved the baculovirus transduction of mammalian cells
核衣壳蛋白VP39融合TAT转导肽对杆状病毒转导哺乳动物细胞的影响

In order to improve the transduction efficiency of insect baculovirus in mammalian cells, we constructed two recombinant baculoviruses, AcRed-tat and AcRed. Both viruses expressed red fluorescence protein gene (dsRed) as a reporter in mammalian cell lines. AcRed-tat also contained the coding sequence of HIV-1 Tat transduction peptide fused with viral major capsid protein gene vp39 and enhanced green fluorescence gene (egfp) driven by virus polyhedrin promoter. It expressed the Tat fusion protein in infected...