%0 Journal Article
%T Tat transduction peptide displayed on the nucleocapsid improved the baculovirus transduction of mammalian cells<br>核衣壳蛋白VP39融合TAT转导肽对杆状病毒转导哺乳动物细胞的影响
%A Li Li
%A Xin Wang
%A Juan Yin
%A Jiang Zhong
%A <br>李俪
%A 王鑫
%A 尹隽
%A 钟江
%J 生物工程学报
%D 2009
%I 
%X In order to improve the transduction efficiency of insect baculovirus in mammalian cells, we constructed two recombinant baculoviruses, AcRed-tat and AcRed. Both viruses expressed red fluorescence protein gene (dsRed) as a reporter in mammalian cell lines. AcRed-tat also contained the coding sequence of HIV-1 Tat transduction peptide fused with viral major capsid protein gene vp39 and enhanced green fluorescence gene (egfp) driven by virus polyhedrin promoter. It expressed the Tat fusion protein in infected...
%K baculovirus
%K mammalian cells
%K transduction
%K Tat transduction peptide<br>杆状病毒
%K 哺乳动物细胞
%K 转导
%K Tat转导肽
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=DE50CEF3FAA6430BA350CBF85E8CF17D&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=F3090AE9B60B7ED1&sid=784CFDF6185AED9D&eid=3F0F3A1477A0E1FE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0