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生物工程学报 2009
Optimize conditions and activities for neutrophil lipase immobilized by nano-silica dioxide
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Abstract:
We used adsorption method to immobilize the lipase by using nano-silica dioxide as the carrier.And we also studied different immobilization conditions effects on the catalytic activity.We got optimize immobilization conditions which were as follow the lipase quantity of 28 300 U/g,temperature of 45oC,pH of 7.5 and treated for 10 h.Under these conditions the immobilized lipase activity yield 3867 U/g carrier.The best reactive temperature for immobilized lipase was 45oC and higher than 5oC for free enzyme,and...