%0 Journal Article
%T Optimize conditions and activities for neutrophil lipase immobilized by nano-silica dioxide<br>二氧化硅纳米材料固定中性脂肪酶的条件优化及其特性
%A Jie Jin
%A Yanhong Yang
%A Ke Wu
%A Hualin Wang
%A Bin Liu
%A Zhimin Yu
%A <br>金杰
%A 杨艳红
%A 吴克
%A 王华林
%A 刘斌
%A 俞志敏
%J 生物工程学报
%D 2009
%I 
%X We used adsorption method to immobilize the lipase by using nano-silica dioxide as the carrier.And we also studied different immobilization conditions effects on the catalytic activity.We got optimize immobilization conditions which were as follow the lipase quantity of 28 300 U/g,temperature of 45oC,pH of 7.5 and treated for 10 h.Under these conditions the immobilized lipase activity yield 3867 U/g carrier.The best reactive temperature for immobilized lipase was 45oC and higher than 5oC for free enzyme,and...
%K nano-silica dioxide
%K immobilization
%K lipase
%K biodiesel<br>二氧化硅纳米材料
%K 固定化
%K 脂肪酶
%K 生物柴油
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=812D5E2F083F7BDE74F5CCCC3E0A4B8A&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=59906B3B2830C2C5&sid=D43C4A19B2EE3C0A&eid=A732AF04DDA03BB3&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18