Metabolic characteristics and kinetic model of recombinant CHO cells in serum-free suspension batch culture
CHO工程细胞无血清悬浮分批培养的生长代谢特征及动力学模型

By using the cell density, cell viability, Pro-UK activity, specific consumption rate of glucose (qglc), specific production rate of lactate (qlac), yield of lactate to glucose (Ylac/glc) and as the evaluation indexes, the growth and metabolism characteristics of pro-urokinase (Pro-UK) expressing CHO cells in serum-free suspension batch culture were examined and compared to those in serum-containing suspension batch culture. We observed hardly differences in growth and metabolism characteristics between the...