%0 Journal Article
%T Metabolic characteristics and kinetic model of recombinant CHO cells in serum-free suspension batch culture<br>CHO工程细胞无血清悬浮分批培养的生长代谢特征及动力学模型
%A Xingmao Liu
%A Hong Liu
%A Lingling Ye
%A Shichong Li
%A Benchuan Wu
%A Haitao Wang
%A Jing Xie
%A Zhaolie Chen
%A <br>刘兴茂
%A 刘红
%A 叶玲玲
%A 李世崇
%A 吴本传
%A 王海涛
%A 谢靖
%A 陈昭烈
%J 生物工程学报
%D 2010
%I 
%X By using the cell density, cell viability, Pro-UK activity, specific consumption rate of glucose (qglc), specific production rate of lactate (qlac), yield of lactate to glucose (Ylac/glc) and as the evaluation indexes, the growth and metabolism characteristics of pro-urokinase (Pro-UK) expressing CHO cells in serum-free suspension batch culture were examined and compared to those in serum-containing suspension batch culture. We observed hardly differences in growth and metabolism characteristics between the...
%K recombinant CHO cells
%K serum-free medium
%K batch culture
%K metabolic dynamics<br>CHO工程细胞
%K 无血清培养基
%K 分批培养
%K 代谢动力学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C501EACCD4F750789954D30ADB7E2AC1&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=CD775AE9DDBD7B53&eid=08805F9252973BA4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0