Rapid site-directed mutagenesis on full-length plasmid DNA by using designed restriction enzyme assisted mutagenesis
用DREAM技术进行全长质粒快速定点突变

To use the designed restriction enzyme assisted mutagenesis technique to perform rapid site-directed mutagenesis on double-stranded plasmid DNA.The target amino acid sequence was reversely translated into DNA sequences with degenerate codons,resulting in large amount of silently mutated sequences containing various restriction endonucleases(REs).Certain mutated sequence with an appropriate RE was selected as the target DNA sequence for designing mutation primers.The full-length plasmid DNA was amplified wit...