%0 Journal Article
%T Rapid site-directed mutagenesis on full-length plasmid DNA by using designed restriction enzyme assisted mutagenesis<br>用DREAM技术进行全长质粒快速定点突变
%A Baozhong Zhang
%A Duoliang Ran
%A Xin Zhang
%A Xiaoping An
%A Yunzhu Shan
%A Yusen Zhou
%A Yigang Tong
%A <br>张宝中
%A 冉多良
%A 张昕
%A 安小平
%A 单云竹
%A 周育森
%A 童贻刚
%J 生物工程学报
%D 2009
%I 
%X To use the designed restriction enzyme assisted mutagenesis technique to perform rapid site-directed mutagenesis on double-stranded plasmid DNA.The target amino acid sequence was reversely translated into DNA sequences with degenerate codons,resulting in large amount of silently mutated sequences containing various restriction endonucleases(REs).Certain mutated sequence with an appropriate RE was selected as the target DNA sequence for designing mutation primers.The full-length plasmid DNA was amplified wit...
%K site-directed mutagenesis
%K polymerase chain reaction
%K designed restriction enzyme ssisted mutagenesis<br>定点突变
%K 聚合酶链反应
%K 设计限制酶辅助突变
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=165EC7AF5B54A2559D27E610D18DF4E4&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=31BCE06A2FD82A16&eid=7737D2F848706113&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=17