Induction of immune response after oral inoculation of mice with Lactobacillus casei surface-displayed porcine epidemic diarrhea viral N protein
表面表达猪流行性腹泻病毒核蛋白蛋白的重组干酪乳杆菌诱导产生的免疫应答

To evaluate the immune responses of recombinant Lactobacillus casei 393 expressing Porcine Epidemic Diarrhea Viral(PEDV) N protein as oral vaccine,n gene of PEDV was subcloned into the expression vector pPG-1,and then transformed into L.casei 393 by electroporation,resulting in recombinant strain pPG-1-n/L.casei 393.The recombinant strains were induced to express interest protein,which was detected by Western blotting,immunofluorescence microscopy and the whole bacteria ELISA.And then BALB/C mice were used ...