%0 Journal Article
%T Induction of immune response after oral inoculation of mice with Lactobacillus casei surface-displayed porcine epidemic diarrhea viral N protein<br>表面表达猪流行性腹泻病毒核蛋白蛋白的重组干酪乳杆菌诱导产生的免疫应答
%A Junwei Ge
%A Yanping Jiang
%A Miao Wang
%A Xinyuan Qiao
%A Min Liu
%A Lijie Tang
%A Yijing Li
%A <br>葛俊伟
%A 姜艳平
%A 汪淼
%A 乔薪瑗
%A 刘敏
%A 唐丽杰
%A 李一经
%J 生物工程学报
%D 2009
%I 
%X To evaluate the immune responses of recombinant Lactobacillus casei 393 expressing Porcine Epidemic Diarrhea Viral(PEDV) N protein as oral vaccine,n gene of PEDV was subcloned into the expression vector pPG-1,and then transformed into L.casei 393 by electroporation,resulting in recombinant strain pPG-1-n/L.casei 393.The recombinant strains were induced to express interest protein,which was detected by Western blotting,immunofluorescence microscopy and the whole bacteria ELISA.And then BALB/C mice were used ...
%K porcine epidemic diarrhea virus
%K N protein
%K Lactobacillus casei
%K immune response<br>猪流行性腹泻病毒
%K N蛋白
%K 干酪乳杆菌
%K 免疫反应
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A4ADB21BEF63F20D94285B6F108C565B&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=FCB110411B6339D8&eid=F2947E14627CD734&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=17