Cloning, expression and functional analysis of the dhbC gene from the siderophore producing bacterium Bacillus subtilis CAS15
枯草芽孢杆菌CAS15嗜铁素基因dhbC的克隆、表达及功能鉴定

We amplified dhbC gene from the siderophore producing bacterium CAS15 by PCR.After ligated the PCR product to pMD18-T vector and then sequenced,we obtained a 1197 bp fragment.The blast result showed that the nucleotide acids of dhbC gene(Accession No.FJ194456) of CAS15 shared 99.7% identity with that of dhbC gene of Bacillus subtilis(GenBank Accession No.Z99120),and was predicted to encode a 43.8 kD polypeptide with 398 amino acid residues.We cloned the dhbC gene into expression vector pET-30a(+) and then t...