%0 Journal Article
%T Cloning, expression and functional analysis of the dhbC gene from the siderophore producing bacterium Bacillus subtilis CAS15<br>枯草芽孢杆菌CAS15嗜铁素基因dhbC的克隆、表达及功能鉴定
%A Xianmei Yu
%A Chao Lin
%A Fucong Zheng
%A Chunping He
%A Xiuguo Zhang
%A <br>余贤美
%A 林超
%A 郑服丛
%A 贺春萍
%A 张修国
%J 生物工程学报
%D 2009
%I 
%X We amplified dhbC gene from the siderophore producing bacterium CAS15 by PCR.After ligated the PCR product to pMD18-T vector and then sequenced,we obtained a 1197 bp fragment.The blast result showed that the nucleotide acids of dhbC gene(Accession No.FJ194456) of CAS15 shared 99.7% identity with that of dhbC gene of Bacillus subtilis(GenBank Accession No.Z99120),and was predicted to encode a 43.8 kD polypeptide with 398 amino acid residues.We cloned the dhbC gene into expression vector pET-30a(+) and then t...
%K Bacillus subtilis
%K dhbC gene
%K gene cloning
%K sequence analysis
%K prokaryotic expression
%K gene knockout<br>枯草芽孢杆菌
%K dhbC基因
%K 基因克隆
%K 序列分析
%K 原核表达
%K 基因敲除
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=BEDE7B4CAF07C6C265A67D8E065BACCA&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=C79A3F06E2AC5B9E&eid=95780E43ADDDE2AA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18