Production of cloned goats by transfer of nuclei from transfected caprine mammary gland epithelial cells
以经过转染的乳腺上皮细胞生产克隆羊

In this study,we evaluated the development potential of caprine mammary gland epithelial cells(CMGECs) after transfection and nuclear transfer into enucleated,ovulated oocytes.We first isolated CMGECs from udders of lactating goats which were transfected with expression plasmid for human lacterrin and selected by G418.Then we chose sixteen neomycin resistant lines and induced them with prolactin for the expression of human lactoferrin checked by Western blotting.The donor cells,expressing human lactoferrin ...