%0 Journal Article
%T Production of cloned goats by transfer of nuclei from transfected caprine mammary gland epithelial cells<br>以经过转染的乳腺上皮细胞生产克隆羊
%A Yuguo Yuan
%A Guoliang Ding
%A Liyou An
%A Junhui Zhao
%A Yujuan Cao
%A Mingxing Miao
%A Yong Cheng
%A <br>袁玉国
%A 丁国梁
%A 安礼友
%A 赵俊辉
%A 曹玉娟
%A 缪明星
%A 成勇
%J 生物工程学报
%D 2009
%I 
%X In this study,we evaluated the development potential of caprine mammary gland epithelial cells(CMGECs) after transfection and nuclear transfer into enucleated,ovulated oocytes.We first isolated CMGECs from udders of lactating goats which were transfected with expression plasmid for human lacterrin and selected by G418.Then we chose sixteen neomycin resistant lines and induced them with prolactin for the expression of human lactoferrin checked by Western blotting.The donor cells,expressing human lactoferrin ...
%K mammary gland epithelial cell
%K goat
%K nuclear transfer
%K human lactoferrin
%K transgene<br>乳腺上皮细胞
%K 山羊
%K 核移植
%K 人乳铁蛋白
%K 转基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=86AFEB54BE7E4BDC5DE7291E43E19204&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=5D311CA918CA9A03&sid=70024E0C77AE588F&eid=CF89D3C02791C82E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=20