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生物工程学报 2009
Metabolic characterization of rat sertoli cell in vitro culture
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Abstract:
Sertoli cell(SC) is intrinsic to the testis and provides an appropriate growth environment for the germ cells.It was separated from rat's testis and identified by hematoxylin and eosin staining(HE) and immunocytochemical reaction, then cultivated in vitro.Culture conditions such as pH, osmotic pressure and metabolic parameters that include consumption rates of glucose, glutamine, amino acids and formation rates of lactic acid, ammonium ion were investigated.It was showed that adhesion process of SCs was acc...