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Metabolic characterization of rat sertoli cell in vitro culture
小鼠睾丸支持细胞体外培养特性

Keywords: sertoli cell,separation and identification,in vitro culture,metabolic characterization
支持细胞
,分离与鉴定,体外培养,代谢特性

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Abstract:

Sertoli cell(SC) is intrinsic to the testis and provides an appropriate growth environment for the germ cells.It was separated from rat's testis and identified by hematoxylin and eosin staining(HE) and immunocytochemical reaction, then cultivated in vitro.Culture conditions such as pH, osmotic pressure and metabolic parameters that include consumption rates of glucose, glutamine, amino acids and formation rates of lactic acid, ammonium ion were investigated.It was showed that adhesion process of SCs was acc...

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