%0 Journal Article
%T Metabolic characterization of rat sertoli cell in vitro culture<br>小鼠睾丸支持细胞体外培养特性
%A Bingyang Shi
%A Shuxiang Zhang
%A Meijin Guo
%A Yonghong Wang
%A Siliang Zhang
%A Xiaolin Shi
%A <br>师冰洋
%A 张淑香
%A 郭美锦
%A 王永红
%A 张嗣良
%A 史小林
%J 生物工程学报
%D 2009
%I 
%X Sertoli cell(SC) is intrinsic to the testis and provides an appropriate growth environment for the germ cells.It was separated from rat's testis and identified by hematoxylin and eosin staining(HE) and immunocytochemical reaction, then cultivated in vitro.Culture conditions such as pH, osmotic pressure and metabolic parameters that include consumption rates of glucose, glutamine, amino acids and formation rates of lactic acid, ammonium ion were investigated.It was showed that adhesion process of SCs was acc...
%K sertoli cell
%K separation and identification
%K in vitro culture
%K metabolic characterization<br>支持细胞
%K 分离与鉴定
%K 体外培养
%K 代谢特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2E403480B9C87F93FFD07364159C8A36&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=94C357A881DFC066&sid=04EA291949415E08&eid=04CF423CEE45E258&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=33