Rescued influenza A virus with codon deoptimized NS1 gene is attenuated both in vitro and in vivo
A型流感病毒NS1基因密码子去优化改造引起病毒毒力减弱

To develop novel live attenuated influenza vaccine, we explored the feasibility to attenuate influenza virus by codon deoptimization of NS1.According to the codon usage bias in influenza A virus, we designed and synthesized a condon-deoptimized NS gene by substituting codons of 110 amino acids in the NS1 gene of A/Puerto Rico/8/34(H1N1) with unpreferred synonymous codons.The influenza A virus with the codon deoptimized NS1 gene(deoNS virus) was rescued by reverse genetics.Plaque forming assay and virus grow...