%0 Journal Article
%T Rescued influenza A virus with codon deoptimized NS1 gene is attenuated both in vitro and in vivo<br>A型流感病毒NS1基因密码子去优化改造引起病毒毒力减弱
%A Shijia Luan
%A Weiqi Pan
%A Ting Li
%A Huaqiang Yang
%A Beiwu Zhang
%A Feng Li
%A Ling Chen
%A <br>栾世家
%A 潘蔚绮
%A 李婷
%A 杨化强
%A 张北武
%A 李锋
%A 陈凌
%J 生物工程学报
%D 2009
%I 
%X To develop novel live attenuated influenza vaccine, we explored the feasibility to attenuate influenza virus by codon deoptimization of NS1.According to the codon usage bias in influenza A virus, we designed and synthesized a condon-deoptimized NS gene by substituting codons of 110 amino acids in the NS1 gene of A/Puerto Rico/8/34(H1N1) with unpreferred synonymous codons.The influenza A virus with the codon deoptimized NS1 gene(deoNS virus) was rescued by reverse genetics.Plaque forming assay and virus grow...
%K influenza A virus
%K NS1 gene
%K codon-deoptimization
%K attenuation
%K live influenza vaccine<br>A型流感病毒
%K NS1
%K 基因
%K 密码子去优化
%K 致弱
%K 减毒活疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6A75CF53410F5CA5800396EDE42D21FD&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=94C357A881DFC066&sid=2A92ABD90588B251&eid=71128DCBD6E39665&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=19