Expression in Escherichia coli, purification and enzymatic properties of porcine urate oxidase
猪尿酸氧化酶在大肠杆菌中的表达、纯化与部分酶学性质分析

The aims of this research were to construct prokaryotic expression vector containing the gene of porcine urate oxidase(pUOX),optimize the conditions of the expression of pUOX in recombinant Escherichia coli BL21(DE3),and analyze the in vitro activity and the enzymological properties of pUOX.The pUOX gene was amplified by RT-PCR from the extracted total RNA of porcine liver,and was inserted into the prokaryotic expression vector pET30a(+) to construct a recombinant expression vector pET30a(+)/pUOX.We identif...