%0 Journal Article
%T Expression in Escherichia coli, purification and enzymatic properties of porcine urate oxidase<br>猪尿酸氧化酶在大肠杆菌中的表达、纯化与部分酶学性质分析
%A Shuanglin Wu
%A Bin Chen
%A Chengqian Liu
%A Yu Ou
%A Jianzhong Yi
%A <br>吴双林
%A 陈斌
%A 刘成倩
%A 欧瑜
%A 易建中
%J 生物工程学报
%D 2009
%I 
%X The aims of this research were to construct prokaryotic expression vector containing the gene of porcine urate oxidase(pUOX),optimize the conditions of the expression of pUOX in recombinant Escherichia coli BL21(DE3),and analyze the in vitro activity and the enzymological properties of pUOX.The pUOX gene was amplified by RT-PCR from the extracted total RNA of porcine liver,and was inserted into the prokaryotic expression vector pET30a(+) to construct a recombinant expression vector pET30a(+)/pUOX.We identif...
%K porcine urate oxidase
%K expression
%K inclusion body
%K purification
%K biological activity<br>猪尿酸氧化酶
%K 表达
%K 包涵体
%K 纯化
%K 生物活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9F4EC5075B2E415F7284CACA1172CA37&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=708DD6B15D2464E8&sid=27B74BA4EDC40A8D&eid=C134FCA51C2CF9D8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=22