A prime-boost vaccination strategy using a Semliki Forest virus replicon vectored DNA vaccine followed by a recombinant adenovirus protects pigs from classical swine fever
猪瘟甲病毒复制子载体DNA疫苗与重组腺病毒活载体疫苗联合免疫效果评价

We have previously evaluated a Semliki Forest virus(SFV) replicon vectored DNA vaccine(pSFV1CS2-E2) and a recombinant adenovirus(rAdV-E2) expressing the E2 glycoprotein of classical swine fever virus(CSFV) in pigs.The results showed that the immunized pigs were protected from virulent challenge, but few pigs showed short-term fever and occasional pathological changes following virulent challenge.To enhance the immunogenecity of the vaccines, we tried a prime-boost vaccination strategy using a combination of...