Overproduction of catalase by oxidative stress on Bacillus subtilis WSHDZ-01
活性氧胁迫促进枯草芽孢杆菌WSHDZ-01过量合成过氧化氢酶

We studied the overproduction of catalase(CAT) by Bacillus sp.WSHDZ-01 by oxidative stress via the feeding of ethanol and the pulse addition of H2O2.By adding 2.0%(V/V) ethanol to the culture broth, the intracellular CAT activity reached 11 151 U/mL, which was 2.5 times than that of the control(4 450 U/mL in flask).By adding 0.3%(V/V) H2O2, more extracellular CAT secreted to the culture broth, and the ratio of extracellular CAT to the total CAT increased to 27%.Based on these results, an oxidative stress st...