%0 Journal Article
%T Overproduction of catalase by oxidative stress on Bacillus subtilis WSHDZ-01<br>活性氧胁迫促进枯草芽孢杆菌WSHDZ-01过量合成过氧化氢酶
%A Dandan Yao
%A Liming Liu
%A Jianghua Li
%A Zhaozhe Hu
%A Guocheng Du
%A Jian Chen
%A <br>姚丹丹
%A 刘立明
%A 李江华
%A 华兆哲
%A 堵国成
%A 陈坚
%J 生物工程学报
%D 2009
%I 
%X We studied the overproduction of catalase(CAT) by Bacillus sp.WSHDZ-01 by oxidative stress via the feeding of ethanol and the pulse addition of H2O2.By adding 2.0%(V/V) ethanol to the culture broth, the intracellular CAT activity reached 11 151 U/mL, which was 2.5 times than that of the control(4 450 U/mL in flask).By adding 0.3%(V/V) H2O2, more extracellular CAT secreted to the culture broth, and the ratio of extracellular CAT to the total CAT increased to 27%.Based on these results, an oxidative stress st...
%K oxidative stress
%K ethanol
%K hydrogen peroxide
%K catalase<br>活性氧胁迫
%K 乙醇
%K 过氧化氢
%K 过氧化氢酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=5332DF66B6B0871CE420F3056288C74E&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=94C357A881DFC066&sid=CFBDB06850C21CC6&eid=1471D37D8144EBCF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=14