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OALib Journal期刊
ISSN: 2333-9721
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Construction of expression vectors for efficient expression of soluble recombinant proteins
高效可溶性重组蛋白表达载体的构建

Keywords: mouse FGF-21,high-level expressive vector,hydroxylamine cleave
鼠源FGF-21
,高效表达载体,羟胺切割

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Abstract:

The aim of the study is to construct two vectors for efficient expression of soluble recombinant proteins. The first vector was constructed by cloning the HisSUMO fragment into an expression vector pET30a(+) to fuse with the gene of interest (designated as HisSUMO Express). The second vector was constructed in the same way, but with a hydroxylamine cleavage site between HisSUMO and the gene of interest for an economic purpose (designated as HisSUMO Economic). The mouse fibroblast growth factor-21(mFGF-21), ...

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