%0 Journal Article
%T Construction of expression vectors for efficient expression of soluble recombinant proteins<br>高效可溶性重组蛋白表达载体的构建
%A Yuanyuan Jiang
%A Mingyao Liu
%A Guiping Ren
%A Huimeng Zhu
%A Deshan Li
%A <br>姜媛媛
%A 刘铭瑶
%A 任桂萍
%A 朱慧萌
%A 李德山
%J 生物工程学报
%D 2010
%I 
%X The aim of the study is to construct two vectors for efficient expression of soluble recombinant proteins. The first vector was constructed by cloning the HisSUMO fragment into an expression vector pET30a(+) to fuse with the gene of interest (designated as HisSUMO Express). The second vector was constructed in the same way, but with a hydroxylamine cleavage site between HisSUMO and the gene of interest for an economic purpose (designated as HisSUMO Economic). The mouse fibroblast growth factor-21(mFGF-21), ...
%K mouse FGF-21
%K high-level expressive vector
%K hydroxylamine cleave<br>鼠源FGF-21
%K 高效表达载体
%K 羟胺切割
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A92204924A25EBB2334746CCA39B39A8&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=CDEBD1ACE0A4C1C1&eid=28F8B56DB6BEE30E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=11