Chronic high dose of insulin stimulates lipolysis in porcine adipocytes
慢性高剂量胰岛素刺激猪脂肪细胞脂肪分解

To explore the effect of chronic high dose of insulin on lipolysis in porcine adipocytes and the underlying molecular regulation mechanisms,we cultured primary porcine adipocytes and incubated them with different concentrations of insulin(0,200,400,800,1600 nmol/L) for 24?96 h in the absence or presence of specific protein kinase A(PKA) inhibitor or extracellular signal-related kinase(ERK) inhibitor.Then,we measured the glycerol release into the culture media as an indicator of the lipolysis,and observed th...