Transformation of Didymella bryoniae mediated by Agrobacterium tumefaciens
农杆菌介导的甜瓜蔓枯病菌遗传转化体系的建立

Gummy stem blight,a plant disease caused by Didymella bryoniae,is one of the major diseases in melon.The disease can seriously reduce melon yield and quality.However,little information is available on the genetics and functional genomics of the fungal pathogen.In this study,we developed an Agrobacterium-mediated transformation system for D.bryoniae by using a universal pathogenic isolate DB11 and the Agrobacterium tumefaciens strain C58C1 carriing plasmid pBIG2RHPH2 harboring the hygromycin B phosphotransfe...