%0 Journal Article %T Transformation of Didymella bryoniae mediated by Agrobacterium tumefaciens
农杆菌介导的甜瓜蔓枯病菌遗传转化体系的建立 %A Haiying Ren %A Li Fang %A Gang Li %A Shuijiang Ru %A Hanrong Wang %A
任海英 %A 方丽 %A 李岗 %A 茹水江 %A 王汉荣 %J 生物工程学报 %D 2010 %I %X Gummy stem blight,a plant disease caused by Didymella bryoniae,is one of the major diseases in melon.The disease can seriously reduce melon yield and quality.However,little information is available on the genetics and functional genomics of the fungal pathogen.In this study,we developed an Agrobacterium-mediated transformation system for D.bryoniae by using a universal pathogenic isolate DB11 and the Agrobacterium tumefaciens strain C58C1 carriing plasmid pBIG2RHPH2 harboring the hygromycin B phosphotransfe... %K Didymella bryoniae %K Agrobacterium mediated transformation %K T-DNA insertional mutagenesis
甜瓜蔓枯病菌,根癌农杆菌介导的转化,T-DNA插入突变 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D1C4D7C9C2701F0659C5065DD8198983&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=B31275AF3241DB2D&sid=4AB2A4AB67F2B16E&eid=15890B67B1F0B7E7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0