Prokaryotic expression and bioreactivity analysis of a major epitope region of 2C with 3AB within non-structural protein of foot-and-mouth disease virus
FMDV非结构蛋白2C主要表位区与3AB基因的组合表达与活性分析

In recent years,the potential value of nonstructural protein(NSP) 2C was well documented for distinguishing foot-and-mouth disease virus(FMDV) in infected animals and vaccinated animals.In order to develop a more sensitive approach to detect natural infected FMDV while there is no interact with vaccinated FMDV,we incorporated a major epitope region of 2C with whole 3AB coding region within NSP and expressed in Escherichia coli.We got a 47.6 kD fusion protein named 2C'3AB.The product showed a specific reacti...