%0 Journal Article %T Prokaryotic expression and bioreactivity analysis of a major epitope region of 2C with 3AB within non-structural protein of foot-and-mouth disease virus
FMDV非结构蛋白2C主要表位区与3AB基因的组合表达与活性分析 %A Xiaoli Zhang %A Meina Tian %A Zengjun Lu %A Yuanfang Fu %A Xiaojun M %A Zaixin Liu %A Qingge Xie %A
张小丽 %A 田美娜 %A 卢曾军 %A 付元芳 %A 马小军 %A 刘在新 %A 谢庆阁 %J 生物工程学报 %D 2009 %I %X In recent years,the potential value of nonstructural protein(NSP) 2C was well documented for distinguishing foot-and-mouth disease virus(FMDV) in infected animals and vaccinated animals.In order to develop a more sensitive approach to detect natural infected FMDV while there is no interact with vaccinated FMDV,we incorporated a major epitope region of 2C with whole 3AB coding region within NSP and expressed in Escherichia coli.We got a 47.6 kD fusion protein named 2C'3AB.The product showed a specific reacti... %K FMDV %K 2C'3AB
非结构蛋白 %K 原核表达 %K 活性分析 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=AA8ACE93FF0DBD02DA4F1A7B87EEE3DE&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=1FAD12DC02D78FFC&eid=8617DE92ECCFAE42&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=16