Introduce Tagsk1 into Salt-sensitive Callus to Improve the Capacity of Salt-tolerance by Micropartical Bombardment
利用基因枪法将Tagsk1基因导入敏盐小麦成熟胚愈伤组织提高其耐盐性的研究

The Tagsk1(Triticum asetium L. glycogen synthase kinase 1)gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1(AF525086). A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed. And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5% NaCl. The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5% NaCl.