%0 Journal Article
%T Introduce Tagsk1 into Salt-sensitive Callus to Improve the Capacity of Salt-tolerance by Micropartical Bombardment<br>利用基因枪法将Tagsk1基因导入敏盐小麦成熟胚愈伤组织提高其耐盐性的研究
%A XU Tao
%A ZHAO Bao-Cun
%A GE Rong-Chao
%A SHEN Yin-Zhu
%A HUANG Zhan-Jing
%A <br>徐涛
%A 赵宝存
%A 葛荣朝
%A 沈银柱
%A 黄占景
%J 生物工程学报
%D 2006
%I 
%X The Tagsk1(Triticum asetium L. glycogen synthase kinase 1)gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1(AF525086). A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed. And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5% NaCl. The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5% NaCl.
%K microparticle bombardment
%K transformation
%K common wheat
%K callus of mature embryos
%K Tagsk1<br>基因枪
%K 转化
%K 小麦
%K 成熟胚愈伤组织
%K Tagsk1
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=47FB6F62177DD11C&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=CC0ECB9C52F1B85F&eid=797D49279EA93BC4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=19