Promoter recognition and b-galactosidase reporter gene expression in Rhodococcus
红球菌启动子识别及b-半乳糖苷酶报告基因表达

The genus Rhodococcus is of considerable interest in recent years,stemming from their diverse applications in biodegradation,bioremediation,biotransformation and biosurfactant.Using Nocardia/Rhodococcus-Escherichia coli shuttle plasmid pNV18.1 as the backbone vector,we tested the driven efficiency of promoters Ptac and PlacZ of E.coli and Pami-1/Pami-2 of R.ruber in host R.rhodochrous ATCC 33278 by overexpression of nitrile hydratase.Results showed that the specific activity of nitrile hydratase per dry cel...