%0 Journal Article %T Promoter recognition and b-galactosidase reporter gene expression in Rhodococcus
红球菌启动子识别及b-半乳糖苷酶报告基因表达 %A Changchun Liu %A Huimin Yu %A Yuchao M %A Wenyu Pan %A Hui Luo %A Zhongyao Shen %A
刘昌春 %A 于慧敏 %A 马玉超 %A 潘雯宇 %A 罗晖 %A 沈忠耀 %J 生物工程学报 %D 2009 %I %X The genus Rhodococcus is of considerable interest in recent years,stemming from their diverse applications in biodegradation,bioremediation,biotransformation and biosurfactant.Using Nocardia/Rhodococcus-Escherichia coli shuttle plasmid pNV18.1 as the backbone vector,we tested the driven efficiency of promoters Ptac and PlacZ of E.coli and Pami-1/Pami-2 of R.ruber in host R.rhodochrous ATCC 33278 by overexpression of nitrile hydratase.Results showed that the specific activity of nitrile hydratase per dry cel... %K Rhodococcus %K driven efficiency of promoter %K nitrile hydratase %K reporter gene %K b-galactosidase
红球菌 %K 启动子启动效率 %K 腈水合酶 %K 报告基因 %K β-半乳糖苷酶 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=59B12985ABA43C85532A41A050CED3ED&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=9CF7A0430CBB2DFD&sid=35D8C930A8A0D2C6&eid=995E04834DE9F169&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=32