Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology
响应面法优化热带假丝酵母104菌株产羰基还原酶发酵培养基

Using response surface method,we optimized the medium for the asymmetric whole cell biotransformation by Candida tropicalis 104.This strain was used for microbial reduction of 1-3,5-bis(trifluoromethyl)phenyl] ethanone to(S)-1-3,5-bis(trifluoromethyl)phenyl] alcohol,with enantiomeric excess(e.e.) reached more than 99.9%.Fractional factorial design was used to evaluate the effects of medium components on carbonyl reductase activity of Candida tropicalis 104.Yeast extract,glucose and NH4Cl were the most imp...