%0 Journal Article
%T Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology<br>响应面法优化热带假丝酵母104菌株产羰基还原酶发酵培养基
%A Pu Wang
%A Liming Sun
%A Junyao He
%A <br>王普
%A 孙立明
%A 何军邀
%J 生物工程学报
%D 2009
%I 
%X Using response surface method,we optimized the medium for the asymmetric whole cell biotransformation by Candida tropicalis 104.This strain was used for microbial reduction of 1-3,5-bis(trifluoromethyl)phenyl] ethanone to(S)-1-3,5-bis(trifluoromethyl)phenyl] alcohol,with enantiomeric excess(e.e.) reached more than 99.9%.Fractional factorial design was used to evaluate the effects of medium components on carbonyl reductase activity of Candida tropicalis 104.Yeast extract,glucose and NH4Cl were the most imp...
%K Candida tropicalis
%K carbonyl reductase
%K (S)-1-[3
%K 5-bis(trifluoromethyl)phenyl] alcohol
%K response surface methodology
%K medium optimization<br>热带假丝酵母
%K 羰基还原酶
%K （S）-1-（3
%K 5-二三氟甲基）苯基乙醇
%K 响应面
%K 培养基优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=71E7202C5A23747BA80FD45287639833&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=AD0A5DE51C29AB9F&eid=B84F2E0A99FDC89A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=6&reference_num=12