Expression and analysis of the extracellular domain of human glucocorticoid-induced tumor necrosis factor receptor ligand in Escherichia coli
人肿瘤坏死因子受体的配体胞外段的原核表达及分析

GITRL(Glucocorticoid-induced tumor necrosis factor receptor ligand) has been recently identified as a novel inhibitor of osteoclastogenesis and hence called Osteostat.In this study, we expressed recombinant extracellular domain of GITRL protein in Escherichia coli and analyzed its bioactivity.Using an Eco31I enzyme-based restriction and ligation method, we obtained an E.coli-preferred DNA sequence coding for the extracellular domain of human GITRL.The DNA was cloned into expression vector pQE-30Xa that enco...