%0 Journal Article
%T Expression and analysis of the extracellular domain of human glucocorticoid-induced tumor necrosis factor receptor ligand in Escherichia coli<br>人肿瘤坏死因子受体的配体胞外段的原核表达及分析
%A Yanli Jiao
%A Fang Zheng
%A Xiaoxia Li
%A Baoli Wang
%A Shanyi Guo
%A <br>焦艳丽
%A 郑纺
%A 李晓霞
%A 王宝利
%A 郭善一
%J 生物工程学报
%D 2009
%I 
%X GITRL(Glucocorticoid-induced tumor necrosis factor receptor ligand) has been recently identified as a novel inhibitor of osteoclastogenesis and hence called Osteostat.In this study, we expressed recombinant extracellular domain of GITRL protein in Escherichia coli and analyzed its bioactivity.Using an Eco31I enzyme-based restriction and ligation method, we obtained an E.coli-preferred DNA sequence coding for the extracellular domain of human GITRL.The DNA was cloned into expression vector pQE-30Xa that enco...
%K GITRL
%K prokaryotic expression
%K reporter gene
%K codon preference
%K restriction enzyme Eco31I<br>GITRL
%K 原核表达
%K 报道基因
%K 偏嗜性
%K 限制性内切酶Eco31I
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6254480B88AA4CAFE95199985B7725C0&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=94C357A881DFC066&sid=C3BC38F6CC09E835&eid=A586B761C9AA2FAA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=9