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生物工程学报 2009
High cell-density fermentation of shark hepatical stimulator analogue in Escherichia coli
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Abstract:
The potential effects of recombinant shark hepatical stimulator analogue(r-sHSA) in liver disease have been revealed in our previous studies.In order to further evaluate its clinic application,we carried out high cell-density fermentation in 5 L fermentor to get enough products.Based on the trials in shaking flask,we optimized the parameters for 5 L fermentor,including medium composition,medium supplement,inducer concentration and induction time,etc.In detail,the improved LB medium(0.97% glycerol,0.91% yeas...
