%0 Journal Article
%T High cell-density fermentation of shark hepatical stimulator analogue in Escherichia coli<br>鲨肝刺激物质类似物在大肠杆菌中的高密度发酵
%A Boping Ye
%A Zheng Pan
%A Huaibiao Li
%A Ying Wang
%A Ying Wang
%A Wutong Wu
%A <br>叶波平
%A 潘征
%A 李怀标
%A 王颖
%A 郑珩
%A 吴梧桐
%J 生物工程学报
%D 2009
%I 
%X The potential effects of recombinant shark hepatical stimulator analogue(r-sHSA) in liver disease have been revealed in our previous studies.In order to further evaluate its clinic application,we carried out high cell-density fermentation in 5 L fermentor to get enough products.Based on the trials in shaking flask,we optimized the parameters for 5 L fermentor,including medium composition,medium supplement,inducer concentration and induction time,etc.In detail,the improved LB medium(0.97% glycerol,0.91% yeas...
%K recombinant shark hepatical stimulator analogue(r-sHSA)
%K high cell density fermentation
%K uniform design
%K biomass
%K expression level<br>重组鲨肝刺激物质类似物
%K 高密度发酵
%K 均匀设计
%K 生物量
%K 表达量
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CF282D6A2AE1A52B57C5D9DB4EF14D4A&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=9CF7A0430CBB2DFD&sid=40DE18199B7CA9BB&eid=9129323FE7AA9847&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18