Development of single base extension-tags microarray for the detection of food-borne pathogens
基于单碱基延伸标签反应的常见食源性致病菌基因芯片检测方法的建立

We developed single base extension-tags (SBE-tags) microarray to detect eight common food-borne pathogens,including Staphylococcus aureus,Vibrio parahaemolyticus,Listeria monocytogenes,Salmonella,Enterobacter sakazaki,Shigella,Escherichia coli O157:H7 and Campylobacter jejuni. With specific PCR primers identified and integrated for eight food-borne pathogens,target sequences were amplified and purified as template DNA of single base extension-tags reaction. The products were hybridized to microarrays and sc...