Soluble expression, purification and activity analysis of extracellular domain III of Flt1
Flt-1胞外Ⅲ区蛋白的可溶性表达、纯化及活性测定

To prepare a soluble human extracellular III domain of Flt1 and analyze its biological activity. The gene encoding extracellular domain III of Flt-1 was cloned into the expression vector pAZY by RT-PCR from human umbilical vein endothelial cell (HUVEC),and induced to express in Escherichia coli by low phosphoric medium,the product was purified by E-tag affinity chromatography. SDS-PAGE and Western blotting analysis showed that Flt-1 gene domain III gene was expressed in E. coli and the yield of the soluble ...