%0 Journal Article
%T Soluble expression, purification and activity analysis of extracellular domain III of Flt1<br>Flt-1胞外Ⅲ区蛋白的可溶性表达、纯化及活性测定
%A Yinliang Xie
%A Yue Gu
%A Rui Huang
%A Xuexia Li
%A Xue Du
%A Jinhong Wang
%A Dongsheng Xiong
%A Chunzheng Yang
%A Yuanfu Xu
%A <br>谢印良
%A 谷岳
%A 黄蕊
%A 李雪霞
%A 杜雪
%A 王金宏
%A 熊冬生
%A 杨纯正
%A 许元富
%J 生物工程学报
%D 2009
%I 
%X To prepare a soluble human extracellular III domain of Flt1 and analyze its biological activity. The gene encoding extracellular domain III of Flt-1 was cloned into the expression vector pAZY by RT-PCR from human umbilical vein endothelial cell (HUVEC),and induced to express in Escherichia coli by low phosphoric medium,the product was purified by E-tag affinity chromatography. SDS-PAGE and Western blotting analysis showed that Flt-1 gene domain III gene was expressed in E. coli and the yield of the soluble ...
%K Flt-1
%K angiogenesis
%K VEGF165<br>血管内皮生长因子受体1
%K 血管新生
%K 血管内皮生长因子165
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=459379ED1D0634E6CE09B732FCB22FCB&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=E158A972A605785F&sid=28B3EB92D5061EA4&eid=CD26609C367AC9C8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14