Ssp DnaB intein-mediated ligation of heavy and light chains of coagulation factor VIII in Escherichia coli
Ssp DnaB intein大肠杆菌中介导FVIII重链和轻链的连接

We studied the ligation of coagulation factor VIII heavy and light chains in Escherichia coli by utilizing the intein-mediated protein trans-splicing. A B-domain deleted factor VIII (BDD-FVIII) gene was broken into two halves of heavy and light chains before Ser1657 which meets the splicing required conserved residue and then fused to 106 and 48 amino acid-containing N-part termed Int-N and C-part termed Int-C coding sequences of split mini Ssp DnaB intein respectively. These two fusion genes were construct...