%0 Journal Article
%T Ssp DnaB intein-mediated ligation of heavy and light chains of coagulation factor VIII in Escherichia coli
Ssp DnaB intein大肠杆菌中介导FVIII重链和轻链的连接
%A Fuxiang Zhu
%A Zelong Liu
%A Huige Qu
%A Xiaolin Xin
%A Hongxin Dong
%A Xiangqin Liu
%A
朱甫祥
%A 刘泽隆
%A 屈慧鸽
%A 辛晓林
%A 董洪新
%A 刘相钦
%J 生物工程学报
%D 2009
%I
%X We studied the ligation of coagulation factor VIII heavy and light chains in Escherichia coli by utilizing the intein-mediated protein trans-splicing. A B-domain deleted factor VIII (BDD-FVIII) gene was broken into two halves of heavy and light chains before Ser1657 which meets the splicing required conserved residue and then fused to 106 and 48 amino acid-containing N-part termed Int-N and C-part termed Int-C coding sequences of split mini Ssp DnaB intein respectively. These two fusion genes were construct...
%K intein
%K protein trans-splicing
%K coagulation factor VIII
Intein
%K 蛋白质反式剪接
%K 凝血VIII因子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F5452988492AA9A2E505C2AB4B7637ED&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=DF92D298D3FF1E6E&sid=C17A97755771D03C&eid=2D52C01434B71375&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=10