The Application of Co-culture System on the in vitro Development of Bovine Somatic Nuclear Transferred Embryos
共培养体系在牛核移植胚体外发育培养中的应用

To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P<0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P<0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P<0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10?F in SOFaa could improve development of nuclear transferred bovine embryo in vitro.