%0 Journal Article
%T The Application of Co-culture System on the in vitro Development of Bovine Somatic Nuclear Transferred Embryos<br>共培养体系在牛核移植胚体外发育培养中的应用
%A WU Yue-Hong
%A AN Zhi-Xing
%A ZHANG Yong
%A LI Xiang-Chen
%A HE Yu-Long
%A LIU Feng-Jun
%A <br>吴月红
%A 安志兴
%A 张涌
%A 李向臣
%A 何玉龙
%A 刘凤军
%J 生物工程学报
%D 2006
%I 
%X To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P<0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P<0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P<0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10?F in SOFaa could improve development of nuclear transferred bovine embryo in vitro.
%K co-culture
%K bovine
%K oviductal epithelial cell
%K nuclear transfer
%K BFF<br>共培养
%K 牛
%K 输卵管上皮细胞
%K 核移植
%K BFF
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=47EFF214F28DAACF&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=31BCE06A2FD82A16&eid=85002451B65CE0D1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=22