Study of Target Pegylated Recombinant Mutant Human Granulocyte Colony Stimulating Factor
聚乙二醇定点修饰重组人粒细胞集落刺激因子突变体的研究

Recombinant mutant human granulocyte colony stimulating factor(rmhG-CSF)was pegylated,purified and characterized.rhG-CSF was mutated in position 1,3,4,5,17,and cysteine was added in C-terminal.rmhG-CSF was pegylated by PEG-Mal 20000 and separated by ion-exchange chromatography,gel filtration chromatography.Analysis of SDS-PAGE showed thar the purity of the separated PEG-rmhG-CSF was greater than 95%.and in intro and in vivo bioactivity study showed that target modified PEG-rmhG-CSF kept full bioactivity which was better than traditional pegylation method,and longer half-life was proved in mice.