%0 Journal Article
%T Study of Target Pegylated Recombinant Mutant Human Granulocyte Colony Stimulating Factor<br>聚乙二醇定点修饰重组人粒细胞集落刺激因子突变体的研究
%A HUANG Yan-Shan
%A JIN Rong
%A LIU Xiao-Ni
%A WANG Chang-Mei
%A RONG Ya-Wen
%A CHEN Zhi
%A <br>黄岩山
%A 金荣
%A 刘晓妮
%A 王昌梅
%A 戎亚雯
%A 陈智
%J 生物工程学报
%D 2007
%I 
%X Recombinant mutant human granulocyte colony stimulating factor(rmhG-CSF)was pegylated,purified and characterized.rhG-CSF was mutated in position 1,3,4,5,17,and cysteine was added in C-terminal.rmhG-CSF was pegylated by PEG-Mal 20000 and separated by ion-exchange chromatography,gel filtration chromatography.Analysis of SDS-PAGE showed thar the purity of the separated PEG-rmhG-CSF was greater than 95%.and in intro and in vivo bioactivity study showed that target modified PEG-rmhG-CSF kept full bioactivity which was better than traditional pegylation method,and longer half-life was proved in mice.
%K pegylation
%K recombinant mutant human granulocyte colony stimulating factor
%K purification
%K characterization<br>聚乙二醇化修饰
%K 重组人粒细胞集落刺激因子突变体
%K 纯化
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EC5FDFA7BEE02169&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=46DFDABDAA819280&eid=3019419AF3C0F2FB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=9