Preparation and bioactivity of anti-human red blood cell ScFv and CSFV E2 bifunctional fusion protein
抗人红细胞单链抗体与猪瘟E2蛋白双功能融合蛋白的构建及生物学活性检测

The aim of this study is to construct a bifunctional fusion protein, which can conjugate both human red blood cells and antibodies against classical swine fever virus (CSFV). We respectively amplified 2E8ScFv and mE2 genes from different recombinant vectors, in which 2E8ScFv gene is the single chain Fv gene against H antigen of human red blood cells, whereas mE2 gene is the main antigen coding region gene of CSFV E2 protein. We used overlap extension PCR to obtain an artificial fusion gene segment 2E8mE2 co...